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The expression of immune defense-related genes in largemouth bass fry fed soybean meal. Immune effector factors (A); immune tolerance-related transcription factors (B, C, and D); and immune signal transduction factors (E and F). There were significant differences indicated by different letters on bars ( P < 0.05), n = 6. FM1: fish meal-based diet for Phase 1; SBM1: 15% soybean meal diet for Phase 1.

Journal: Animal Nutrition

Article Title: Nutritional programming through early exposure to soybean meal: Effects on growth performance, intestinal health, metabolism, and microbiota of juvenile largemouth bass ( Micropterus salmoides )

doi: 10.1016/j.aninu.2025.11.005

Figure Lengend Snippet: The expression of immune defense-related genes in largemouth bass fry fed soybean meal. Immune effector factors (A); immune tolerance-related transcription factors (B, C, and D); and immune signal transduction factors (E and F). There were significant differences indicated by different letters on bars ( P < 0.05), n = 6. FM1: fish meal-based diet for Phase 1; SBM1: 15% soybean meal diet for Phase 1.

Article Snippet: RNA samples with a concentration greater than 100 ng/μL and an OD 260 /OD 280 ratio between 1.8 and 2.2 were considered to have acceptable integrity. cDNA synthesis was performed using a reverse transcription kit (PC7002, Aidlab Bio Inc., Beijing, China).

Techniques: Expressing, Transduction

The expression of immune defense-related genes in largemouth bass fed soybean meal was mean ± SEM ( n = 4). Immune effector factors (A); immune tolerance-related transcription factors (B, C, and D); and immune signal transduction factors (E and F). Significant differences were observed between different letters on bars ( P < 0.05). F1F2: fish meal-based diet for Phase 1 (FM1) followed by fish meal-based diet for Phase 2 (FM2; F1S2: FM1 followed by 25% soybean meal diet for Phase 2 (SBM2); S1F2: 15% soybean meal diet for Phase 1 (SBM1) followed by FM2; S1S2: SBM1 followed by SBM2.

Journal: Animal Nutrition

Article Title: Nutritional programming through early exposure to soybean meal: Effects on growth performance, intestinal health, metabolism, and microbiota of juvenile largemouth bass ( Micropterus salmoides )

doi: 10.1016/j.aninu.2025.11.005

Figure Lengend Snippet: The expression of immune defense-related genes in largemouth bass fed soybean meal was mean ± SEM ( n = 4). Immune effector factors (A); immune tolerance-related transcription factors (B, C, and D); and immune signal transduction factors (E and F). Significant differences were observed between different letters on bars ( P < 0.05). F1F2: fish meal-based diet for Phase 1 (FM1) followed by fish meal-based diet for Phase 2 (FM2; F1S2: FM1 followed by 25% soybean meal diet for Phase 2 (SBM2); S1F2: 15% soybean meal diet for Phase 1 (SBM1) followed by FM2; S1S2: SBM1 followed by SBM2.

Article Snippet: RNA samples with a concentration greater than 100 ng/μL and an OD 260 /OD 280 ratio between 1.8 and 2.2 were considered to have acceptable integrity. cDNA synthesis was performed using a reverse transcription kit (PC7002, Aidlab Bio Inc., Beijing, China).

Techniques: Expressing, Transduction